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mouse vegf elisa kits  (Multi Sciences (Lianke) Biotech Co Ltd)


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    Multi Sciences (Lianke) Biotech Co Ltd mouse vegf elisa kits
    Metformin promotes type H vessel formation in OVX mice and elevates endothelial angiogenesis in vitro under hypoxic conditions. ( A-B ) Representative Ki67 and EMCN co-immunostaining images ( A ) with quantification of the number of Ki67 positive endothelial cells (Ki67 + ECs, B ). gp: growth plate. tb: trabecular bone. bm: bone marrow. Scale bar: 200 μm. n = 4 per group. ( C-D ) Representative CD31 and EMCN co-immunostaining images ( C ) with quantification of the ratio of CD31 hi EMCN hi vessel ( D ) in femoral metaphysis from Sham, Sham + Metf, OVX and OVX + Metf groups. Scale bar: 200 μm. n = 4–5 per group. ( E ) <t>ELISA</t> for the serum and bone marrow concentrations of VEGFA. n = 4–5 per group; ( F ) CCK-8 analysis for the proliferation of metformin-treated HMECs under different oxygen concentrations (1% and 21% O 2 ). n = 4 per group; ( G-H ) Representative images ( G ) of tube formation of HMECs on Matrigel with quantification of the total tube length, total loops and total branching points ( H ) in (G). Scale bar: 100 μm; n = 3 per group. Data are plotted as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001
    Mouse Vegf Elisa Kits, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Metformin ameliorates osteoporosis by enhancing bone angiogenesis via the YAP1/TAZ-HIF1α axis"

    Article Title: Metformin ameliorates osteoporosis by enhancing bone angiogenesis via the YAP1/TAZ-HIF1α axis

    Journal: Molecular Medicine

    doi: 10.1186/s10020-025-01169-7

    Metformin promotes type H vessel formation in OVX mice and elevates endothelial angiogenesis in vitro under hypoxic conditions. ( A-B ) Representative Ki67 and EMCN co-immunostaining images ( A ) with quantification of the number of Ki67 positive endothelial cells (Ki67 + ECs, B ). gp: growth plate. tb: trabecular bone. bm: bone marrow. Scale bar: 200 μm. n = 4 per group. ( C-D ) Representative CD31 and EMCN co-immunostaining images ( C ) with quantification of the ratio of CD31 hi EMCN hi vessel ( D ) in femoral metaphysis from Sham, Sham + Metf, OVX and OVX + Metf groups. Scale bar: 200 μm. n = 4–5 per group. ( E ) ELISA for the serum and bone marrow concentrations of VEGFA. n = 4–5 per group; ( F ) CCK-8 analysis for the proliferation of metformin-treated HMECs under different oxygen concentrations (1% and 21% O 2 ). n = 4 per group; ( G-H ) Representative images ( G ) of tube formation of HMECs on Matrigel with quantification of the total tube length, total loops and total branching points ( H ) in (G). Scale bar: 100 μm; n = 3 per group. Data are plotted as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001
    Figure Legend Snippet: Metformin promotes type H vessel formation in OVX mice and elevates endothelial angiogenesis in vitro under hypoxic conditions. ( A-B ) Representative Ki67 and EMCN co-immunostaining images ( A ) with quantification of the number of Ki67 positive endothelial cells (Ki67 + ECs, B ). gp: growth plate. tb: trabecular bone. bm: bone marrow. Scale bar: 200 μm. n = 4 per group. ( C-D ) Representative CD31 and EMCN co-immunostaining images ( C ) with quantification of the ratio of CD31 hi EMCN hi vessel ( D ) in femoral metaphysis from Sham, Sham + Metf, OVX and OVX + Metf groups. Scale bar: 200 μm. n = 4–5 per group. ( E ) ELISA for the serum and bone marrow concentrations of VEGFA. n = 4–5 per group; ( F ) CCK-8 analysis for the proliferation of metformin-treated HMECs under different oxygen concentrations (1% and 21% O 2 ). n = 4 per group; ( G-H ) Representative images ( G ) of tube formation of HMECs on Matrigel with quantification of the total tube length, total loops and total branching points ( H ) in (G). Scale bar: 100 μm; n = 3 per group. Data are plotted as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001

    Techniques Used: In Vitro, Immunostaining, Enzyme-linked Immunosorbent Assay, CCK-8 Assay



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    Image Search Results


    Metformin promotes type H vessel formation in OVX mice and elevates endothelial angiogenesis in vitro under hypoxic conditions. ( A-B ) Representative Ki67 and EMCN co-immunostaining images ( A ) with quantification of the number of Ki67 positive endothelial cells (Ki67 + ECs, B ). gp: growth plate. tb: trabecular bone. bm: bone marrow. Scale bar: 200 μm. n = 4 per group. ( C-D ) Representative CD31 and EMCN co-immunostaining images ( C ) with quantification of the ratio of CD31 hi EMCN hi vessel ( D ) in femoral metaphysis from Sham, Sham + Metf, OVX and OVX + Metf groups. Scale bar: 200 μm. n = 4–5 per group. ( E ) ELISA for the serum and bone marrow concentrations of VEGFA. n = 4–5 per group; ( F ) CCK-8 analysis for the proliferation of metformin-treated HMECs under different oxygen concentrations (1% and 21% O 2 ). n = 4 per group; ( G-H ) Representative images ( G ) of tube formation of HMECs on Matrigel with quantification of the total tube length, total loops and total branching points ( H ) in (G). Scale bar: 100 μm; n = 3 per group. Data are plotted as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001

    Journal: Molecular Medicine

    Article Title: Metformin ameliorates osteoporosis by enhancing bone angiogenesis via the YAP1/TAZ-HIF1α axis

    doi: 10.1186/s10020-025-01169-7

    Figure Lengend Snippet: Metformin promotes type H vessel formation in OVX mice and elevates endothelial angiogenesis in vitro under hypoxic conditions. ( A-B ) Representative Ki67 and EMCN co-immunostaining images ( A ) with quantification of the number of Ki67 positive endothelial cells (Ki67 + ECs, B ). gp: growth plate. tb: trabecular bone. bm: bone marrow. Scale bar: 200 μm. n = 4 per group. ( C-D ) Representative CD31 and EMCN co-immunostaining images ( C ) with quantification of the ratio of CD31 hi EMCN hi vessel ( D ) in femoral metaphysis from Sham, Sham + Metf, OVX and OVX + Metf groups. Scale bar: 200 μm. n = 4–5 per group. ( E ) ELISA for the serum and bone marrow concentrations of VEGFA. n = 4–5 per group; ( F ) CCK-8 analysis for the proliferation of metformin-treated HMECs under different oxygen concentrations (1% and 21% O 2 ). n = 4 per group; ( G-H ) Representative images ( G ) of tube formation of HMECs on Matrigel with quantification of the total tube length, total loops and total branching points ( H ) in (G). Scale bar: 100 μm; n = 3 per group. Data are plotted as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001

    Article Snippet: According to the manufacturer’s instructions, the concentrations of VEGFA were determined by commercial Mouse VEGF ELISA Kits (Multi Sciences LTD., Hangzhou, China).

    Techniques: In Vitro, Immunostaining, Enzyme-linked Immunosorbent Assay, CCK-8 Assay